PW01-023 – Ex vivo PBMC cytokine profile in FMF patients

Results Ex vivo cytokine profile The levels of pro-inflammatory cytokines IL-6 and TNF-a were higher in unstimulated and LPS stimulated PBMC supernatants of FMF patients in crises compared to the control group. Concentrations were comparable between FMF patients during and between crises. There was no difference in spontaneous IL1-b and IL-1 a release by PBMCs of FMF patients and controls. However, in response to LPS stimulation, levels of these cytokines were found higher in PBMC supernatants of FMF patients during crises compared to those in remission and to the controls. In contrast, no difference was found in IL1-RA levels between FMF patients and controls in all conditions. Regarding Th1 and Th2 cytokines, IFN-g and IL-4 levels were lower in unstimulated and anti-CD3/CD28 stimulated PBMCs supernatants of FMF patients during and between crises compared to the controls. Moreover, lower levels of those cytokines were detected in culture supernatants of FMF patients during crises compared to those in remission after T cell stimulation. For Th17 cytokines, IL-17 was higher in anti-CD3/ CD28 stimulated PBMC supernatants of FMF patients during crises compared to the control group. After T cell stimulation, PBMCs from FMF patients in remission release more IL-22 than PBMCs from control subjects. Finally, no difference in IL-10 levels was detected between FMF patients and controls.